In order to evaluate the effect of glue penetration on cigarette filter ventilation rate, and provide the basis for tipping glue and tipping paper selection, using the PVC rectangular pore pressure plate control the area of the original coating area, calculate the penetration area of coloring glue using pixel method, estsblished a method to measure the natural permeability of the tipping glue, and the natural permeability of 2 kinds of tipping glue on 12 kinds of tipping paper was measured and analyzed. The results showed that: ① the natural permeability of 2 kinds of tipping glue on 12 kinds of tipping paper was between 0.024 5%～0.138 0% and 0.011 8%～0.112 0%, and RSD was less than 10%; ② The ANOVA results showed that the types of tipping glue and tipping paper had a very significant effect on the natural permeability; ③ K-means clustering analysis results showed that the proper combination of tipping glue and tipping paper would help reduce the natural permeability of glue.