东北市售鸡肉中空肠弯曲杆菌的分离鉴定、分型及耐药性分析
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(1. 河南科技大学食品与生物工程学院,河南 洛阳 471023;2. 长白山食品药品检验检测中心,吉林 白山 134500;3. 穆棱市食品检验检测中心,黑龙江 牡丹江 157500 )

作者简介:

费鹏,女,河南科技大学讲师,博士。

通讯作者:

康怀彬(1963—),男,河南科技大学教授,硕士。E-mail:khbin001@163.com

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基金项目:

河南科技大学博士科研启动基金项目(编号:13480066);河南省重点攻关项目 (编号:161100110900)


Isolation,identification,multilocus sequence typing analysis and antimicrobial resistance of Campylobacter jejuni isolated from commercial chicken in northeast China
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(1. College of Food and Biological Engineering, Henan University of Science and Technology, Luoyang, Henan 471023, China; 2. Changbai Mountain Food and Drug Inspection Testing Center, Baishan, Jilin 134500, China; 3. MuLeng City Food Inspection Testing Center, Mudanjiang, Heilongjiang 157500, China)

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    摘要:

    为了揭示东北地区市售鸡肉中空肠弯曲杆菌的污染情况、种群特征及耐药性。采集东北地区市售鸡肉样品1 000份,分离鉴定空肠弯曲杆菌。通过多位点序列分型技术分析空肠弯曲杆菌的遗传多样性。利用K-B琼脂扩散法检测该致病菌对8种抗生素的耐药性。结果表明:1 000份样品中共分离出62株空肠弯曲杆菌,污染率为6.2%;62株空肠弯曲杆菌被分为14个序列型(Sequence Type,ST),其中ST5和ST1为该种群的优势ST;耐药性分析结果显示,62株空肠弯曲菌对环丙沙星、复方新诺明和头孢噻肟具有高度的敏感性。

    Abstract:

    In order to reveal the contamination, population characteristics and antimicrobial resistance of Campylobacter jejuni isolated from commercial chicken in Northeast China, 1 000 chicken samples were collected from the Northeast China. C. jejuni was isolated and identified.The genetic diversity of C. jejuni was analyzed by multilocus sequence typing (MLST). The antimicrobial resistance of C. jejuni to 8 antibiotics was detected by K-B AGAR diffusion method. The results indicated that 62 C. jejuni strains were isolated from 1 000 chicken samples with a contamination rate of 6.2%, and were divided into 14 sequence types (STs). Among them, ST1 and ST5 were the dominant STs. The results of antimicrobial resistance analysis showed that 62 C. jejuni strains were highly sensitive to ciprofloxacin, compound sinomin and cefotaxime.

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费鹏,赵胜娟,姜亦超,等.东北市售鸡肉中空肠弯曲杆菌的分离鉴定、分型及耐药性分析[J].食品与机械,2018,34(6):46-49.
FEIPeng, ZHAOShengjuan, JIANGYichao, et al. Isolation, identification, multilocus sequence typing analysis and antimicrobial resistance of Campylobacter jejuni isolated from commercial chicken in northeast China[J]. Food & Machinery,2018,34(6):46-49.

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  • 收稿日期:2018-02-25
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  • 在线发布日期: 2023-03-17
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