Based on solid phase extraction (SPE) and ultra high performance liquid chromatography (UPLC) technique, a method has been developed for the simultaneous detection and analysis of 6 fluoroquinolones residues in aquatic products. The optimized process and parameters of this method were as followed: treated aquatic products were extracted with phosphate buffer, the extracting solution were purified by SPE and concentrated by blowing nitrogen, and the residues were then dissolved in 0.2% formic acid solution for UPLC analysis; Chromatographic column BEH C₁₈ (1.7 μm, 2.1 mm×50 mm) were applied, and citric acid (0.05 mol/L)-ammonium acetate (0.1 mol/L) buffer were used as the mobile phase with 0.42 mL/min. the sample volume was 0.2 μL, column temperature was 50 degree centigrade, and the excitation and emission wave length of fluorescence detector were 278 nm and 465 nm, respectively. The proposed method is simple and effective, with recovery rate range between 63.69% and 90.83%, limit of detection range between 0.5 and 1.8 μg/kg, and relative standard deviation less than 10%.