As the temperature control system of beer fermentation has the characteristics of time-varying, nonlinear and lagging, the traditional control method can not be used to control the beer fermentation temperature accurately. In this paper, the variable universe fuzzy PID controller was designed by introducing the extension and reduction factors of the universe, and the PID parameters were on-line fine tuning. Based on S7-1200 controller and KTP1000 touch screen, the automatic control system of beer fermentation was developed to achieve human-machine interaction. The results of beer fermentation test showed that: the control error of beer fermentation temperature ±0.09 ℃, the control accuracy 0.75%. The tempera-ture control system designed has good stability and high accuracy, which can increase the quality of beer.