Rice was inoculated with spore suspension of Aspergillus flavus, and far-infrared radiation (FIR) was used to study the disinfection on curve and toxin production ability of A. flavus in rice with different water contents. Moreover, the effect on the quality of rice color, free amino acids, and soluble protein was considered. The results showed that the decontamination of FIR radiation on A. flavus was significantly enhanced with the increase of temperature and time. When the rice moisture content was 30%, FIR heating at 115 ℃ for 5 minutes resulted in 2.96±0.28-log reductions of A. flavus spores in rice. The total amount of aflatoxin B₁ (AFB₁) and the amount of AFB₁ produced by unit cell decreased by 63.09% and 38.44%, respectively. When the moisture content of rice was 20%, FIR heating resulted in 2.04 ± 0.17-log reductions. The total amount of AFB₁ was decreased by 55.04% and the detoxification by unit cell was 22.54%. After FIR heating at 115 ℃ followed tempering at 70 ℃, the logarithmic amount of residual spores was more than 3.87. L value, b value, △E and free amino acid content had no significant difference. The content of soluble protein of rice decreased with the increasing of treatment time.