2-L-Alanine-2-deoxy-D-Glucose(Ala-Glu) was synthesized from L-Alanine(Ala) and D-fructose(Fru) through condensation dehydration and rearrangement reaction. The thermal weight loss and pyrolysis properties of Ala-Glu were investigated by thermogravimetry-derivative thermogravimetry(TG-DTG) and pyrolysis gas chromatography-mass spectroscopy(Py-GC/MS). Using the moisture content (d.b.) of tobacco shred as an indicator, the physical moisture retention properties of Ala-Glu were measured. The effect of these compounds on sensory comfort of cigarette was investigated. The results show that: ① The synthetic product was the target compound; ② The primary pyrolysis temperature of Ala-Glu was 178.9 ℃, and the thermal mass loss reached about 90% at 800 ℃. The number of pyrolytic products of the target compound increased with the rise of temperature, and the major pyrolytic products of Ala-Glu were including pyrazine, pyridine, pyrrole, furan, pyrone; ③ Moisture retention of Ala-Glu was better than that of propylene glycol and glycerin; ④ Ala-Glu can make mainstream cigarette smoke mellow and full, reduce irritation and miscellaneous odor, and have a role to enhance on cigarette sensory comfort.