The leaf crude extracts were obtained from Gingo biloba L. using ethanol extraction method, and its preservation on litchi was studied on the aspects of color, V_C content, weight loss rate, the total number of bacteria and sensory evaluation. The results showed that leaf crude extract of ginkgo biloba could reduce the loss of water, inhibit the browning, reduce the decrease of V_C content, the infection of litchi flesh and the decay rate of litchi, and keep the sensory quality of litchi. Its ability of antibacterial effects and preservation was followed by 0.6% leaf crude extract of ginkgo biloba＞0.4% leaf crude extract of ginkgo biloba＞0.2% leaf crude extract of ginkgo biloba＞control. It indicated that the positive correlation was found between the preservation effect on litchi and the concentration of crude extract of ginkgo biloba leaves within a certain range. Theerefore, it was found that the leaf crude extract of ginkgo biloba had better preservation effect on litchi.