It is an important method to break rubber seeds with the principle of collision. In order to study the breaking mechanics of rubber seeds in the collision process, the instantaneous impact force of rubber seeds was measured by RFP data acquisition system. In this experiment, single factor method was adopted to select the height, angle and initial velocity factors to analyze the influence of various factors on the impact force of rubber seeds. The experimental results showed that (the perspective of the range is 0° to 90°) : When height increased from 0.5 m to 2.0 m, the collision force increased from 1.712 to 3.461 N. When the Angle increases from 30 degrees to 90 degrees, the impact force increases from 0.831 N to 2.401 N. When the initial velocity increased from 0.5 to 5.0 m/s, the impact force increased from 1.781 to 3.311 N. Through the SPSS software was used to estimate the curve and fitting out the optimal model, and it couuld concluded that the height, angle and initial velocity were positively correlated with the impact force, and the correlation was significant. The results of homogeneity test showed that the variance of the three groups was homogeneity in height, initial velocity and angle. According to the experimental data, the formula was derived by multiple linear regression analysis. The results can be combined in different cases of the size of the rubber seed crushing force, which provide a test to design the reasonable structure of cutting, enabling the machine to complete the shell work more efficiently.