The polysaccharides from the seeds and peel of Sophora japonica L. were extracted respectively by double enzymes, The basic structure of polysaccharides were identified by using infrared spectroscopy,and antioxidant activity, moisture absorption and retention capacities of polysaccharides were evaluated. The results indicated that the polysaccharides extraction yield of Sophora japonica L. seeds and peel were 11.6% and 24.7% respectively. The IR spectrum indicated peel and seed polysaccharides had typical characteristic absorption peaks of polysaccharides and pyran ring structure, seed polysaccharides contained a-glycosidic bonds, peel polysaccharides contained α and β glycosidic bonds.The radical scavenging experiment showed that two different polysaccharides of Sophora japonica L. had a certain radical scavenging capacity. Within a certain concentration range, the radical scavenging activity of seeds polysaccharides was better than that of peel polysaccharides, and had the positive correlations with concentration of polysaccharides. Compared with the positive control vitamin C(V_C), the ABTS and DPPH radical scavenging ability of two polysaccharides were weak, but the scavenging effect of seeds polysaccharide on superoxide anion was better than that of V_C. Compared with the positive control EDTA, determination of two polysaccharides on ferrous ion chelating ability were weak. In the same conditions, moisture absorption capacities of two polysaccharides of sophora fructus were close to that of chitosan but lower than that of glycerin. Moisture retention capacities of two polysaccharides of sophora fructus were equivalent, lower than glycerin, but higher than chitosan. These results demonstrated that polysaccharides of Sophora japonica L. had good radical scavenging activity and moisture retention capacity which had the development and utilization value.