Clove oil was loaded into β-Cyclodextrin (β-CD) using the saturated aqueous solution method. Antibacterial film was prepared by adding the microcapsule into Soy Protein Isolate (SPI) film, microcapsule embedding ratio, the mechanical properties of films and the bacteriostatic action to Bacillus subtilis and Escherichia coli were determined. The results showed that the embedding ratio was （67.42±3.82）%; the effect of bacteriostatic action to Bacillus subtilis and Escherichia coli of film adding more microcapsule was better，but the mechanical properties were not very well.