A horseradish peroxidase enzyme-sensitized detection platform based on functionalized magnetic nanoparticles and gold nanoparticles was established with the nanomaterials as the carrier and the bisphenol A aptamer and the complementary strand as the biometric recognition unit. The method about rapid pretreatment and trace detection of bisphenol A in food matrix was established. The BPA aptamer binding to the magnetic nanomaterials could compete with the complementary strands of the aptamer and the horn root peroxidase (HRP) modified gold nanoparticles. The effect of HRP on the catalytic hydrolysis of the substrate resulted in the change of characteristic peaks at 450 nm, helping to quantitatively detect bisphenol A, which had the advantages of good selectivity, high sensitivity and good specificity. The results showed that this detection system had a linear relationship (R²=0.978 4) in the concentration range of 0～100 ng/mL, and the limit of detection was as low as 0.5 pg/mL. This work provideed strong technical support for detection of bisphenol A in future.