In this study, 2 strains of Enterococcus faecalis HYD09 and HYN06 isolated from traditional fermented food were used to determine the enzyme activity of fermentation supernatant, and evaluate the basic safety of the strains. The safer strain were tested for gastrointestinal viability; meanwhile, the fermentation supernatant was subjected to protease; the crude enzyme was obtained from the fermentation broth and its activity was measured by Native-Page. The results showed: the enzyme activities of HYD09 and HYN06 fermentation supernatant were 115.3 U/mL and 107.2 U/mL, respectively; HYD09 and HYN06 indole test, nitrate reductase assay, amino acid decarboxylase test hemolysis test were negative, no multiple antibiotic resistance to 14 kinds of antibiotics. Moreover, no virulence gene was detected in strain HYD09, while the virulence gene esp was detected in strain HYN06. This confirmed that HYD09 strains were safe. Further studies showed that the HYD09 had the ability of acid resistance and bile salt tolerance; besides, the fermentation supernatant of strain HYD09 still had fibrinolytic activity after pepsin and trypsin treatment. Two main components were found in the crude enzyme of the fermentation supernatant of HYD09 strain, and one of them had fibrinolytic activity.