Lactic acid bacteria (LAB) with ability of acid and bile salt resistance were isolated from the feces of the longevity population in Rugao by using selective medium, artificial gastric juice and bile salt medium, and the ability of cholesterol reducing and manner were studied. The results showed that the survival rate of all isolated 60 strains of LAB in the pH 3.0 artificial gastric juice was more than 30%, the LAB from the middle-aged group, elderly group and longevity group was significantly higher than that in the youth group (P＜0.05); the survival rate of LAB in the 0.3% and 0.5% bile salt culture medium was more than 20%, of which the LAB in elderly group and longevity group was significantly higher than that of youth group and middle-aged group (P＜0.05). The ability of cholesterol lowering of the LAB from the longevity group was significantly higher than that of middle-aged group (P＜0.05), the ability of cholesterol reducing of lactobacillus spp. and Weissella spp. was significantly higher than that of Enterococcus spp. (P＜0.05), the main manners of cholesterol lowering of LAB were cell absorption and sedimentation. The LAB come from longevity population have the strong ability of acid and bile salt resistance and cholesterol lowering, and the longevity group are higher than others from other age groups.