提取方法对天麻多糖提取率及其抗氧化活性的影响
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(四川农业大学农学院,四川 成都 611130)

作者简介:

王庆,男,四川农业大学在读硕士研究生。

通讯作者:

吴卫(1970—),女,四川农业大学教授,博士生导师,博士。E-mail:ewuwei@sicau.edu.cn

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四川省科技支撑计划子课题(编号:2014SZ0131)


Effect of different extraction methods on the extraction ratio and antioxidant activity of polysaccharides from Gastrodia elata Bl.
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(Agronomy College, Sichuan Agricultural University, Chengdu, Sichuan 611130, China)

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    摘要:

    分别采用热水浸提、超声辅助提取和酶法提取对四川平武乌天麻多糖进行提取,并对天麻多糖提取率及其抗氧化化活性进行比较,为筛选适合天麻多糖提取的方法提供参考。结果表明:3种提取方法对天麻多糖提取率及其抗氧化活性影响较大,其中酶法提取天麻多糖提取率最高(为50.315%),与其他2种提取方法间差异达到极显著水平(P<0.01);在DPPH和FRAP法抗氧化评价体系中,多糖抗氧化活性表现为超声辅助提取>酶法提取>热水浸提法,而在ABTS抗氧化评价体系中,其活性顺序是酶法提取>超声辅助提取>热水浸提。综合分析,超声辅助提取和酶法提取有利于天麻多糖提取,且能保持其高效的抗氧化活性。

    Abstract:

    In order to screen a suitable extraction method for polysaccharides from Gastrodia elata Bl., the hot water extraction, ultrasound extraction and enzymatic hydrolysis were applied to extracting polysaccharides from G. elata Bl.f. glauca S. Chow from Pingwu in Sichuan province, then the polysaccharides extraction ratios and their antioxidant activities were compared. The polysaccharides extraction ratios and antioxidant activities were obviously influenced by the extraction methods. Of them, the extraction ratio of enzymatic hydrolysis was the highest with the value of 50.315%, which was extremely different from the other extraction methods (P<0.01). The antioxidant activities were in the following order: ultrasound extraction, enzymatic hydrolysis and hot water extraction by using DPPH free radical scavenge capacity and FRAP values. However, the antioxidant activities were in the following order: enzymatic hydrolysis, ultrasound extraction and hot water extraction by using ABTS free radical scavenge capacity. In conclusion, due to the higher extraction rate and antioxidant activity, ultrasound extraction and enzymatic hydrolysis were efficient to extract polysaccharides from G. elata.

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引用本文

王庆,李丹丹,潘芸芸,等.提取方法对天麻多糖提取率及其抗氧化活性的影响[J].食品与机械,2017,33(9):146-150.
WANGQing, LIDandan, PANYunyun, et al. Effect of different extraction methods on the extraction ratio and antioxidant activity of polysaccharides from Gastrodia elata Bl.[J]. Food & Machinery,2017,33(9):146-150.

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  • 在线发布日期: 2023-03-10
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