传统泡菜中抗性乳酸菌的筛选及鉴定
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(1. 西南大学食品科学学院,重庆 400715;2. 食品科学与工程国家级实验教学示范中心〔西南大学〕,重庆 400715;3. 西南大学重庆市特色食品工程技术研究中心,重庆 400715)

作者简介:

刘思雨,女,西南大学在读本科生。

通讯作者:

索化夷(1978—),男,西南大学副教授,硕士生导师,博士。E-mail:birget@swu.edu.cn

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基金项目:

重庆市社会民生科技创新专项(编号:cstc2015 shmszx80021);重庆市特色食品工程技术研究中心能力提升项目(编号:cstc2014ptgc8001);中央高校基本业务费专项资金(编号:XDJK2016A018)


Screening and identification of resistant lactobacillus in traditional pickles
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(1. College of Food Science in Southwest University, Chongqing 400715, China; 2. National Demonstration Center for Experimental Food Science and Technology Education, Chongqing 400715, China; 3. Chongqing Engineering Research Center of Regional Food, Chongqing 400715, China)

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    摘要:

    以传统泡菜中分离出的40 株乳酸菌为对象,通过耐酸、抗人工胃液和抗胆盐试验,确定传统泡菜中具有良好抗性的乳酸菌,并使用16S rDNA序列和系统发育树分析良好抗性乳酸菌的种属关系。试验结果表明,40 株乳酸菌中编号MFR-28和MFR-30两株菌对人工胃液和胆盐具有较强的耐受能力,其在人工胃液中的存活率分别为85.21%和9047%,在0.3%胆盐中的生长效率分别为11.84%和737%;经16S rDNA序列和系统发育树分析,MFR-28和MFR-30均为干酪乳杆菌(Lactobacillus casei)。该研究筛选出的2 株抗性干酪乳杆菌,可作为潜在益生菌进一步探索其生理功能,以期用于开发益生菌产品。

    Abstract:

    40 strains of actic acid bacteria isolated by traditional pickled cabbage were regarded as the object. Through the tests of acid resistance, anti gastric juice and bile salt resistance, actic acid bacteria with good resistance in traditional pickles were determined, using 16S rDNA sequence and phylogenetic tree to analyze the genus relationship of good resistance of lactic acid bacteria. The experimental results showed that MFR-28 and MFR-30 facing to artificial gastric juice and bile salt had a strong tolerance in 40 strains of actic acid bacteria, and survival rates in the artificial gastric juice were 85.21% and 90.47% respectively, and the growth of efficiency in 0.3% bile salt were 11.84% and 7.37% respectively; through the analysis of 16S rDNA sequence and phylogenetic tree, MFR-28 and MFR-30 were Lactobacillus casei. In this study, 2 strains of resistant lactobacillus casei can be used as potential probiotics to further explore their physiological functions and to develop probiotic products.

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引用本文

刘思雨,肖菁,索化夷.传统泡菜中抗性乳酸菌的筛选及鉴定[J].食品与机械,2017,33(7):26-30.
LIUSiyu, XIAOJing, SUOHuayi. Screening and identification of resistant lactobacillus in traditional pickles[J]. Food & Machinery,2017,33(7):26-30.

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  • 在线发布日期: 2023-03-10
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