金银花等6种植物提取物总黄酮含量与抗氧化性相关性研究
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(山东理工大学农业工程与食品科学学院,山东 淄博 255000)

作者简介:

付晶晶,女,山东理工大学在读硕士研究生。

通讯作者:

肖海芳(1980—),女,山东理工大学讲师,博士。E-mail:xiaohaifang@sdut.edu.cn

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基金项目:

山东省自然科学基金青年项目(编号:ZR2014CQ002)


Acomparative study of contents of total flavonoids and their antioxidant activities in six plants
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(School of Agricultural Engineering and Food Science, Shandong University of Technology, Zibo, Shandong 255000, China)

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    摘要:

    以金银花、核桃叶、南非醉茄、何首乌、红景天、石榴皮6种提取物为试验对象,通过硝酸铝—亚硝酸钠比色法测定了总黄酮含量;采用ABTS、DPPH与Fenton法测定了其抗氧化能力并进行比较;采用Pearson法进行相关性分析。结果表明:对DPPH自由基的清除能力顺序为金银花>核桃叶>南非醉茄>红景天>何首乌>石榴皮;对羟基自由基的清除能力顺序为金银花>核桃叶>南非醉茄>何首乌>石榴皮>红景天;对ABTS+自由基清除能力顺序为金银花>核桃叶>红景天>南非醉茄>何首乌>石榴皮。总黄酮含量对DPPH、ABTS+与羟基自由基清除能力的相关系数分别为0.819,0.848,0.791,表明总黄酮含量与清除DPPH、ABTS+自由基能力呈显著相关性,与清除羟基自由基能力相关性不显著。

    Abstract:

    In the present study, six total flavonoids extracts were prepared from Honey suckle, Walnut leaves, Withania Somnifera, Rhodiola rosea, Pomegranate bark, and Polygonum multiflorum. The total flavonoids content of six plant extracts were measured by aluminium nitrate-sodium nitrite colorimetry. The antioxidant activities of six extracts were compared by the detection methods of DPPH, ABTS and Fenton, and then the correlation of total flavonoids content was analyzed by Pearson. The results showed that the DPPH scavenging order was Honey suckle>Walnut leaves>W. Somnifera>R. rosea>P. multiflorum>Pomegranate bark. The hydroxyl radical scavenging order was Honey suckle>Walnut leaves>W. Somnifera>P. multiflorum>Pomegranate bark>Rhodiola rosea. The results showed that the ABTS+ scavenging order was Honey suckle>Walnut leaves>R. rosea>W. Somnifera>P. multiflorum>Pomegranate bark. The free radical scavenging ability and flavonoids content of six kinds of extracts showed quantitative relationship. The correlation that the total flavonoid content and DPPH, ABTS+ was significantly positive, while with hydroxyl radical was not the same. The correlation coefficients above were 0.819, 0.848 and 0.791, respectively.

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付晶晶,肖海芳,宋元达.金银花等6种植物提取物总黄酮含量与抗氧化性相关性研究[J].食品与机械,2017,33(6):159-163.
FUJingjing, XIAOHaifang, SONGYuanda. Acomparative study of contents of total flavonoids and their antioxidant activities in six plants[J]. Food & Machinery,2017,33(6):159-163.

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  • 在线发布日期: 2023-03-10
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