The typical bacterial membrane in the food industry (Staphylococcus aureus film) was chose to explore the single bacterial membrane in the formation of bacteria, and the production of their own activities with redox activity during the membrane formation and growth process was investigated. Comparing with the real-time changes of the bacterial membrane, the change of the redox activity was also studied to find the mechanism of controlling the bacterial pollution in the food industry. The real-time monitoring of catalase in Staphylococcus aureus membrane was carried out by analyzing the peak current obtained from the bacterial membrane of different culture periods. The results showed that the peroxidation of Staphylococcus aureus changes in the amount of catalase. The amount of catalase in the bacteria increases with the increase of the amount of bacterial membrane, and decreases with the autolysis of the membrane. This study would provide a theoretical and research basis for the study of oxidative metabolism in Staphylococcus aureus.