To investigate the effect of microwave-two enzymes synergistic extraction methods on dietary fiber yields in lotus shell, the sieving mesh number of the lotus seed shell, ratio of solid-liquid, papain and glucoamylase dosages and extraction time were set out to conduct the orthogonal tests based on the results of single factor experiments. The optimal extraction conditions were as followed: sieving mesh of 60, ratio of liquid-solid 201, papain dosage 3%, papain extraction time of 6×20 s (extracting 6 times, each time for 20 s with intervals of 2 min), dosage of glucoamylase 4%, and glucoamylase extraction time 8×20s (extracting 8 times, each time for 20 s with intervals of 2 min). Under the optimal conditions, the extraction rate of dietary fiber in lotus shell was 72.36%. Compared with enzyme extraction, microwave-two enzymes synergistic extraction can save 70% time, and greatly improve the extraction rate over 82.87% than two enzyme method.