This paper compared the pros and cons, the operability of two methodology, by using the Fenlin reagent titration and the 3,5-dinitrosalicylic acid colorimetry, to determine the reducing sugar content and the total sugar content in the wild Jerusalem artichoke from Xiangxi. The RSD value is 2.00% by using Fenlin reagent titration and the precision is 8%~13%, while, the RSD value is 1.68% by using 3,5-dinitrosalicylic acid colorimetry and the precision is 2.5%~4.0%. It is concluded that the Fenlin reagent titration method is susceptible to the finish measurement etc factors, especially for samples with low sugar content, but is more suitable for detection of sample with color, while the 3,5-dinitrosalicylic acid colorimetric method is simple, fast and suitable for batch processing, the RSD value is 0.55% when the color reaction system is in 135 min, the color reaction system is quite stable, little effect on the low concentration sample, and the result is more accurate.