A new method for the determination of selenium（Se） in vegetables and fruits was developed using electric membrane extraction. The effects of several factors were investigated, such as Time spent, Consumption of reagents and Detection limit of sample. The results showed that the detection limit of sample was 0.005 mg/kg by spending few time and reagent. The recovery of Se was 92.4% on an average. Through the verification of the national standard reference materials, the experimental results meet the requirements of the uncertainty. The method is suitable for the detection of selenium in fruits and vegetables.