Bee pollen soluble dietary fiber was extracted by enzymatic extraction, and the physicochemical properties was analyzed, while, the products were compared with the bee pollen soluble dietary fiber by acid extraction and alkali extraction. The optimal enzymatic extraction conditions were determined as follows: extraction temperature 50 ℃, extraction time 2 h, enzyme 2.5%, pH 4. Under the conditions, the yield of bee pollen soluble dietary fiber was 4.11%. The product is milk-white powder, and has good smell. The swelling capacity, water holding capacity and the water-solubility of the fiber were 2.03 mL/g, 1.01 g/g, 91.26%, respectively. The bee pollen soluble dietary fiber was composed of 7 kinds of monosaccharide, such as mannose, rhamnose, glucuronic acid, galacturonic acid, glucose, galactose and arabinose, and has the absorption peak of polysaccharides. Compared with alkali extraction and acid extraction, The bee pollen soluble dietary fiber has better water solubility and sensory quality, and is easier to apply enzymatic extraction to the beverage products development.