The total content of polyphenols in polygonum captitatum was determined by Folin-Cioculteu colorimetry. 10 types of macroporous resins were tested by static adsorption and desorption experiments for purifying polyphenols in polygogum captitatum and purifying conditions by the best resin were investigated. The results showed that HPD-722 was the best resin. Purifying conditions were as follows: concentration of adcorption sample 2.0～4.5 mg/mL, 2 BV/h adsoption velocity, 7.5 BV 50% ethanol solution as eluent, the content of the total polyphenol sample was up to 59.02%.