Objective: The effects of hemp seed and algae oils preparation on blood lipid-lowering and antioxidant activity of mixed hyperlipidemia rats were investigated in this study. 50 rats were randomly divided into 5 groups, i.e., the blank control group, model control group, hemp seed oil and algal oil preparation low dose group (170 mg/kg), medium dose group (340 mg/kg) and high dose group (510 mg/kg). Then their weights of , testis fat pad and perirenal fat pad, fat body ratio, and the activities of malondialdehyde (MDA), glutathione (GSH) and superoxide dismutase (SOD) were measured after feeding high fat diet and corresponding drugs. The results indicated that the weights of gain, and testis and perirenal fat pads, fat body ratio, TG, TC and MDA of model control group rats increased significantly, however, the activities of GSH, GSH-Px and SOD, and the high-density lipoprotein cholesterol (HDL-C) reduced significantly (P < 0.05, P < 0.01), compared to the blank group. Moreover, in the high dose and low dose group of hemp seed oil and algae oil preparation, rats weight, weights of testis and perirenal fat pads, body fat ratio decreased significantly, and the content of MDA decreased significantly, while the activities of SOD, GSH and GSH PX significantly increased (P < 0.05 or P < 0.05), compared to the model control group. In conclusion, our results confirmed that the hemp seed and algae oils preparation could regulate blood lipid and possessed strong antioxidant activities.