In order to regulate the technical conditions of irradiation sterilization for semi-finished Micropterus salmoides (SFMS), we investigated the impacts of irradiation on the microbe quantity, pH value, water holding capacity, color value and volatile components of it in this study, by using different doses ranging from 0.00 to 8.74 kGy of ⁶⁰Co gamma ray irradiation. The results showed that, with the irradiation of 3.25 kGy, the total bacteria decreased to 10 CFU/g. Moreover, the total bacteria decreased to 250 CFU/g and 10 CFU/g, at the D₁₀ value of 1.54 kGy and 3.64 kGy, respectively. At the range of 1.55~4.78 kGy, pH value could be controlled in a reasonable standard. Increasing irradiation dosage (1.55~7.57 kGy) did not change the production of water holding capacity, color, or Warner-Bratzler shear force. A dose of 3.25 kGy was beneficial to semi-finished products of SFMS to reduce the volatile components of benzene and alkanes. In conclusion, the ⁶⁰Co-γ irradiation dose in 3.25~4.78 kGy could release the sterilization purpose. Therefore, our studies provided theoretical guidance for the irradiation sterilization of SFMS.