In order to understand the effect of cigarette quality on the different application amount in different grades of cigarette lap adhesive, six gradient sizing of three different cigarette lap adhesive glue quantity have been tested in the same condition. The results showed that：1）the different cigarette lap adhesive glue quantity was influential of the sensory quality of cigarette, when the amount of the sizing agent was more than 1.5 mL per thousand cigarette, the cigarette sensory quality became worse with the increase of the amount of the glue, mainly manifested in the cigarette sensory stimulation increases, the miscellaneous gas and dry sense increases, the clean degree becomes bad. 2) Effect of the amount of glue on the appearance quality of cigarette, when the sizing value of 1# was 2.0 mL per thousand cigarette, the phenomenon of explosion occurs, when the glue amount was 3.5 mL per thousand cigarette, there was the phenomenon of inner overflow; when the sizing value of 2# and 3# was 1.0 mL per thousand cigarette, the phenomenon of explosion occurs, when the glue amount was 3.5 mL per thousand cigarette, there was the phenomenon of inner overflow. 3) Combined with the evaluation results of the quality of cigarettes inside and outside, it was determined that the amount of the 1# adhesive was 2.5-3.0 mL per thousand cigarette, and 2# or 3# was 1.5 to 2.0 mL per thousand cigarette. Fourth, with the change of cigarette lap adhesive glue quantity, physical quality, chemical components of mainstream smoke of cigarette and seven kinds of harmful ingredients were not obvious.