天麻多酚的超声辅助提取工艺优化及抗氧化活性研究
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(1. 陕西理工大学陕西省资源生物重点实验室,陕西 汉中 723000;2. 陕西理工大学生物科学与工程学院,陕西 汉中 723000;3. 国家农产品保鲜工程技术研究中心秦巴地区保鲜工作站,陕西 汉中 723000;4. 陕南秦巴山区生物资源综合开发协同创新中心,陕西 汉中 723000)

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孙海燕(1979—),女,陕西理工大学讲师,硕士。E-mail:diyson2008@163.com

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陕西省教育厅专项(编号:16JK1155);秦巴山区生物资源综合开发协同创新中心项目(编号:QBXT-Z(P)15-22)


Optimization of process extraction of polyphenols from Gastrodia elata BI and its antioxidant activity
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(1. Shaanxi Sci-tech University Shaanxi Key Laboratory of Resource Biology, Hanzhong, Shaanxi 723000, China; 2. Shaanxi Sci-tech University of Biological Science and Engineering, Hanzhong, Shaanxi 723000, China; 3. National Engineering Research Center for Preservation of Agricultural Products in Qinba Area Preservation Workstation, Hanzhong, Shaanxi 723000, China; 4. Qinling-Bashan Mountains Bioresources Comprehensive Development C. I. C., Hanzhong, Shaanxi 723000, China)

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    摘要:

    以鲜天麻为试材,研究了天麻总酚的超声辅助提取工艺条件,以最佳工艺对不同干燥条件下的天麻粉进行总酚提取,测定其总酚含量与抗氧化能力,并分析两者的相关性。结果表明,影响天麻总酚提取效果的最主要因素是提取温度,最优工艺条件为:提取温度50 ℃、提取时间35 min、提取功率80 W,料液比1∶10(g/mL),在该条件下,天麻总酚含量最高为5.62 mg/g;不同干燥条件下,55 ℃热风干燥制得的天麻粉总酚含量最高,达5.754 mg/g,其总抗氧化能力、抗超氧阴离子自由基能力、抑制羟自由基能力和DPPH自由基清除能力也最高,同时,天麻总酚含量与其抗氧化能力呈显著相关,说明天麻总酚具有一定的抗氧化能力。

    Abstract:

    The fresh Gastrodia elata was used to extract total phenolic content which help to explore an ultrasonic assisted extraction technology.. The total phenol from the G. elata under different drying conditions was extracted by the optimized technology. The total phenolic content and antioxidant capacity were detected, and then the correlation between them was analyzed. The results showed that extraction temperature was the main factor that influenced the extraction efficiency of total phenol. The optimal process condition was.extracting the total phenol for 35 min at 50℃, using a solid-liquid ratio is 110(g/mL)with extraction power 80 W, and consenquently 5.62 mg/g of total phenole was obtained from G. Elata. Furthemor, it was found that the highest content of total phenolic content is 5.754 mg/g in hot air drying among all the different drying conditions, and total antioxidant capacity, anti superoxide anion free radical, hydroxyl radical scavenging activity and DPPH radical scavenging ability of it were also the highest. In addition, the total phenolic content and antioxidant capacity were found significantly correlated with each other, and this indicated that the total phenolic compounds might poccess certain antioxidant capacity.

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孙海燕.天麻多酚的超声辅助提取工艺优化及抗氧化活性研究[J].食品与机械,2016,32(10):158-163.
SUNHaiyan. Optimization of process extraction of polyphenols from Gastrodia elata BI and its antioxidant activity[J]. Food & Machinery,2016,32(10):158-163.

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  • 收稿日期:2016-06-07
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  • 在线发布日期: 2023-03-09
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