芋头多糖提取工艺优化及其体外结合脂类和胆酸盐能力研究
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(江苏农牧科技职业学院食品科技学院,江苏 泰州 225300 )

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刘萍,女,江苏农牧科技职业学院副教授,博士。

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泰州市农业科技支撑项目(编号:TN2013001);江苏省青蓝工程科技创新团队培养对象


Optimization of polysaccharide extraction from taro and its binding capacities of lipid and bile salts in vitro
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(School of Food Science and Technology, Jiangsu Agri-Animal Husbandry Vocational College, Taizhou, Jiangsu 225300, China)

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    摘要:

    以新鲜龙香芋为原料,对其水溶性多糖的水溶醇沉提取工艺进行优化,并通过体外模拟人体胃肠消化环境,测定其对脂肪、胆固醇、胆酸盐的结合能力,考察芋头多糖的体外降脂效果。结果表明,当提取温度80 ℃、提取时间6 h、料液比1∶6(g/mL)、乙醇体积分数85%时,芋头多糖提取效果最好,提取率达(4.92±0.18)%。芋头多糖经过胃肠消化系统后,对脂肪和胆酸盐的吸附效果优于对照纤维素,对两者的结合能力与其质量浓度呈现显著相关性;对胆固醇的吸附效果低于纤维素,表明芋头多糖具有一定的降脂功能,降脂机理与纤维素不同。

    Abstract:

    The extraction technology of water–soluble polysaccharides in fresh taro was investigated in this paper. Using simulated conditions of human digestion in vitro, its fat-binding, cholesterol-binding and bile salts-binding capacities were studied in order to evaluate its potential hypocholesterolemic activity in vitro. The results showed that the highest extraction efficiency of taro polysaccharide could be obtain under the conditon of using solid-liquid ratio at 1∶6, reacting at 80 ℃ for 6 h with 85% ethanol, and the extraction ratio reaches (4.92±0.18)%. Taro water–soluble polysaccharides exhibited much higher binding capacities of both fat and bile acid, while much lower one of cholesterol, compared to cellulose. The above results revealed that taro water–soluble polysaccharides could potentially alleviate the hyperlipidemia.

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刘萍,祁兴普,刘靖,等.芋头多糖提取工艺优化及其体外结合脂类和胆酸盐能力研究[J].食品与机械,2016,32(10):132-136,199.
LIUPing, QIXingpu, LIUJing, et al. Optimization of polysaccharide extraction from taro and its binding capacities of lipid and bile salts in vitro[J]. Food & Machinery,2016,32(10):132-136,199.

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  • 收稿日期:2016-08-29
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  • 在线发布日期: 2023-03-09
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