For the purpose of joint production of hydrolysis of porcine blood and microbial ecology preparation of Clostridium butyricum, it was studied that the solid medium formulation suitable for the growth and reproduction of C. butyricum was made of porcine blood powder, and the fermentation products would be used in feed. The formulation of the solid medium formulation was designed and optimized by the mean of mixture design. Moreover, the mathematical regression model of three components of solid medium was established, and the various components and the interaction between them were also discussed. Furthermore, the results showed that the optimum formula was constituted by the growth promoting factor 4.8%, corn flour 46.3%, porcine blood powder 46.3%. On this condition，the number of C. butyricum was expected to reach 8.18×10⁷ CFU/g, and the content of free amino acid nitrogen increased from 0.02 g/100 g to 3.13 g/100 g during the fermentation. Consequently, variance analysis and verification test both showed that the mathematical model established by mixture design method was suitable for optimizing the solid fermentation of porcine blood powder medium for C. butyricum.