In order to obtain the efficient secretion of intracellular product in yeast, as the original strain,Saccharomyces cerevisiae (2n), (n) were mutagenized with UV treatment, five mutants strain may have temperature-sensitive were selected. To further identify, Y₁ was used as the control bacterium, and its content of extracellular nucleic acids, proteins and FDP were determined, find that the highest degree of autolysis was Ts₄(2n), it’s content of nucleic acid and protein in extracellular environment were 1.709, 1.483, the concentration of FDP in extracellular environment was 38μg/mL, higher than Y₁ 24.1%. Ts^*₂ (n) and Ts^*₃ (n) also have a certain extent of temperature-sensitive. Therefore, 3 temperature-sensitive mutants were obtained in this study, which are Ts₄ (2n), Ts^*₂(n) and Ts^*₃ (n).