核桃青皮多糖微波辅助提取工艺及抗氧化活性研究
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(1. 西南林业大学西南山地森林资源保育与利用省部共建教育部重点实验室,云南 昆明 650224;2. 西南林业大学云南省森林灾害预警与控制重点实验室,云南 昆明 650224;3. 江西师范大学国家单糖化学合成工程技术研究中心,江西 南昌 330022)

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张雪春,女,西南林业大学讲师,博士。

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云南省教育厅科学研究基金重点项目(编号:2014Z105);西南林业大学博士科研启动基金(编号:111303);云南省森林灾害预警与控制重点实验室开放基金(编号:ZK150013)


Study on microwave-assisted extraction and antioxidant activity of polysaccharides from Walnut husks
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(1. Key Laboratory of Conservation Southwest Mountain College of Forestry and Utilization of Forest Resources, Southwest Forestry University, Kunming, Yunnan 650224, China; 2. Key Laboratory of Forest Disaster Warning and Control in Yunnan Province, Southwest Forestry University, Kunming, Yunnan 650224, China; 3. National Research Center for Carbohydrate Synthesis, Jiangxi Normal University, Nanchang, Jiangxi 330022, China)

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    摘要:

    采用响应面分析法优化微波辅助提取核桃青皮多糖工艺,并通过与抗坏血酸对比·OH、DPPH·、ABTS+·的清除能力和总抗氧化能力,评价核桃青皮多糖抗氧化活性。结果表明:微波辅助提取核桃青皮多糖的最佳条件为水料比251(mL/g )、微波功率750 W、提取时间8.5 min、提取次数2次,在该条件下核桃青皮多糖得率可达10.17%。抗氧化试验结果显示,核桃青皮多糖具有一定的抗氧化活性,但弱于抗坏血酸。核桃青皮多糖对·OH、DPPH·、ABTS+·的IC50值分别为83 975.520,628.800,694.733 μg/mL,总抗氧化能力的FRAP值为120.42 μmol/mL。

    Abstract:

    The response surface methodology was used to optimize microwave-assisted extraction process of polysaccharides from walnut husks. Compared with the ascorbic acid, the preliminary antioxidant activities of the polysaccharides was evaluated by investigated the ·OH, DPPH·, ABTS+· free radical scavenging capacity and total antioxidant capacity. The results showed that the optimal conditions were water-feed ratio 25∶1(mL/g), microwave power 750 W, extraction time 8.5 min, extract 2 times. Under these conditions, the yield of polysaccharides was 10.17%. The results of anti-oxidation experiment showed that the walnut husks polysaccharides had some scavenging effects, but not as good as ascorbic acid. The walnut husks polysaccharides scavenged the ·OH, DPPH·, ABTS+· free radical as IC50 values of 83 975.520, 628.800 and 694.733 μg/mL, and the FRAP(total antioxidant capacity) were 120.42 μmol/mL.

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张雪春,田智宇,王振兴,等.核桃青皮多糖微波辅助提取工艺及抗氧化活性研究[J].食品与机械,2016,32(7):146-151.
ZHANGXuechun, TIANZhiyu, WANGZhenxing, et al. Study on microwave-assisted extraction and antioxidant activity of polysaccharides from Walnut husks[J]. Food & Machinery,2016,32(7):146-151.

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  • 在线发布日期: 2023-03-09
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