The quercetin in the 80% ethanol extract of lotus seed pod was identified with UV, TLC, IR and HPLC, and its biology activity was also studied. Results: That the alcohol extract of lotus seed pod contained quercetin; Taking the V_C and tea polyphenol as positive control, the ·OH, O^-₂·, DPPH and ABTS⁺ scavenging activity of 92.02%, 98.54%, 93.12% and 91.32%, respectively, when the concentration of quercetin in the 80% ethanol extract of lotus seed pod reached 8.6583 mg/mL; The MIC of the ethanol extract of lotus seed pod to escherichia coli, yeast and Salmonella was 0.8 mg/mL (quercetin concentration), to staphylococcus aureus was 0.6 mg/mL (quercetin concentration), but didn’t show obvious inhibitory effect on mould. The alcohol extract of lotus seed pod has antioxidant properties and antibacterial properties, and has positive correlation to the concentration of quercetin (R>0.980 0, P<0.05).