Aimed at the severe contamination of AFB₁ in corn, effect of specific gravity separation on AFB₁-contaminated corn has been investigated. In this study, air volume, longitudinal gradient and amplitude have a significant influence on separation results. With air volume 6 000 m³/h, longitudinal gradient 7°, amplitude 15 mm, different levels of contaminated corn were separated with specific gravity. AFB₁ concentrations in obtained heavy corn were decreased by 21.2% and 38.4% respectively compared with the materials. The ratio of light corn and broken percentage of corn was moderate. As the specific gravity equipment is very significant in grain production, using specific gravity method to decrease AFB₁ in corn is practical and promising.