The process of polyphenols ultrasonic-assisted extraction from okra marc by ethanol was studied. Under the condition of ultrasonic power was set at 250 W, four groups of single factor tests were designed to investigate the effects of ratio of material to liquid, ethanol concentration, temperature and duration time on the ultrasonic extraction results of polyphenol from Okra marc. Box-Behnken experimental module in Design-Expert 8.0.6 software was used to design for the combination test of 3 factors, establishment of polynomial regression, response surface analysis and optimization by combination of conditions. Results: Under the conditions of ultrasonic power was 250 W, and the ratio of material to liquid was 25 mL:1 g, the optimized technological parameters for ultrasonic-assisted ethanol to extract polyphenol from Okra marc were: ethanol concentration 57.58%, temperature 51.16 ℃, and duration time 88.40 min, and the yields of extraction rate was 11.72 mg/g, which was the same as the prediction value in the model.