A new method for discrimination of wild Mushroom by means of near infrared spectroscopy (NIRS) was established. Visible and near infrared reflectance spectra of 13 different types of mushroom including 3 wild mushrooms were collected with a portable near infrared spectrometer ( ASD Fieldspec3 ). Based on the analysis of spectrum, are the characteristics absorption peaks of polysaccharides, protein, fat of the mushroom are1 483, 1 727, 1 930, 2 100, 2 180, 2 310 nm, which of wild mushroom in are significantly higher than other mushrooms. With these 6 wavelengths of absorbance as input variables to establish recognition model of support vector machine, which takes RBF kernel, the parameters of C = 2 048.0, gamma = 0.031 25, the model for unknown sample recognition correct was 95.3%. Results show that, using the mushroom sample in a few wavelengths of near infrared spectral characteristics of absorption, establish support vector machine recognition model can be identified very well.