新疆传统发酵酸驼乳优势乳酸菌粘附性质及益生特性研究
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(新疆师范大学生命科学学院,新疆 乌鲁木齐 830054)

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努丽艳·阿不都米力克,女,新疆师范大学在读硕士研究生。

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国家自然科学基金地区基金项目(编号:31160333)


Study on cell adhesion and probiotic characteristics of dominant lactic
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(College of Life Science, Xinjiang Normal University, Urumqi, Xinjiang 830054, China)

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    摘要:

    以从新疆传统发酵酸驼乳优势乳酸菌中筛选出的4株经初步鉴定能耐酸耐胆盐的乳酸菌菌株为研究对象,选取人体肠上皮细胞株Caco-2,采用显微观察和平板计数的方法研究这4株菌对细胞的粘附性质;采用高效液相色谱法进行体外降胆固醇能力研究;并研究了4株乳酸菌对指示菌的抑菌能力。结果显示,Le2-1(Leuconostoc lactis strain)、M4-3-2(Enterococcus sp.)、M3-1(Enterococcus faecium strain)、M3-3(Lactobacillus brevis strain)都具有较强的粘附能力,其中M3-1的粘附能力最强。4种菌株体外胆固醇降解率在28%~41%(M4-3-2的降解率达到40%以上),并且对指示菌都具有较强的抑菌活性。

    Abstract:

    Adopting alternative culture 4 bacteria trains among the superior bacteria were screened from Xinjiang camel acid -milk. Identified as lactic acid bacteria, and own high ability of resistance to acid and salt. The adhensive ability of the strains to cell line Caco-2 was tested via morphological observation and cell counting under microscope. The amount of cholesterol assimilated by the bacteria was measured by high performance liquid chromatography ( HPLC). As results, Le2-1 (Leuconostoc lactis strain), M4-3-2(Enterococcus sp.), M3-1 (Enterococcus faecium strain), M3-3 (Lactobacillus brevis strain) were adhensive to Caco-2. M3-1 was the most adhesive strain among the four tested strains.The cholesterol assimilated range from 28%~41%, M4-3-2 which Assimilated more than 40% Cholesterol from culture medium. The antibacterial capacities of 4 strains were studied. The result showed that the metabolins of 4 strains were of obvious antibacterial activity.

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努丽艳·阿不都米力克,沙吉坦穆·克依斯尔,阿依古扎·依布拉音,等.新疆传统发酵酸驼乳优势乳酸菌粘附性质及益生特性研究[J].食品与机械,2016,32(4):42-46.
NuliyanAbudumilike, SajitanmuKeyisier, AyiguzhaYibulayin, et al. Study on cell adhesion and probiotic characteristics of dominant lactic[J]. Food & Machinery,2016,32(4):42-46.

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  • 收稿日期:2016-01-15
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  • 在线发布日期: 2023-03-09
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