四川腊肉中乳酸菌的筛选、产酸特性及其氨基酸产物分析
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(1. 四川理工学院生物工程学院,四川 自贡 643000;2. 肉类加工四川省重点实验室,四川 成都 610106)

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刘有晴(1990-),女,四川理工学院在读硕士研究生。E-mail:15196006977@163.com

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肉类加工四川省重点实验室重点项目(编号:14-R02);四川理工学院学科建设工程资助项目 (编号:2013TS13)


Screening of lactic acid bacteria from Sichuan bacon and analysis on its acid
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(1. School of Biotechnology Engineering, Sichuan University of Science and Engineering, Zigong, Sichuan 643000, China; 2. Sichuan Province Key Laboratory of Meat Processing, Chengdu, Sichuan 610106, China)

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    摘要:

    对四川腊肉中乳酸菌进行筛选,研究其产酸特性及氨基酸产物。利用可培养方法,对四川腊肉中乳酸菌进行分离筛选,利用16S rDNA的序列测定技术对其鉴定,并对分离菌株在不同温度、pH、NaCl浓度、亚硝酸浓度条件下的生长代谢、产酸能力进行研究及发酵产物中氨基酸成分进行测定分析。结果显示:分离菌株为植物乳杆菌(Lactobacillus paraplantarum),分离菌株对数生长期为6~18 h;最适产酸作用温度范围为30~40 ℃;最适产酸作用pH范围为5.5~6.0;对NaCl及NaNO2耐受能力分别达6.0%和150 mg/L;分离菌株释放游离氨基酸11种,其中必需氨基酸占总氨基酸含量的45.76%,可为后续该植物乳杆菌在发酵肉类中的应用提供基础研究数据。

    Abstract:

    The lactic acid bacteria were screened in Sichuan bacon, and the acid production characteristics and amino acid products were also stydied. Using culture methods, lactic acid bacteria were isolated and screened from Sichuan bacon, and identified by 16S rDNA sequencing technique. Studied the growth metabolism and acid production capacity on the different temperature, pH, NaCl concentration, nitrite concentration and fermentation products of amino acid. The results showed that the isolation strain was Lactobacillus paraplantarum. Through conditions of producing acid and free amino acid analysis, logarithmic growth phase of strain was 6~18 h; the optimum fermentation acid temperature range was 30~40 ℃; optimum fermentation acid pH range was 5.5~6.0; had good endurance abilities to the salt concentration 6.0% and the nitrite content 150 mg/L; this strain had 11 kinds of free amino acid, and the essential amino acids accounted for 45.76% of total amino acid content. This study could provide basic research data for the application of Lactobacillus paraplantarum in fermentation meat products.

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引用本文

刘有晴,黄丹,于华,等.四川腊肉中乳酸菌的筛选、产酸特性及其氨基酸产物分析[J].食品与机械,2016,32(1):34-37.
LIUYouqing, HUANGDan, YUHua, et al. Screening of lactic acid bacteria from Sichuan bacon and analysis on its acid[J]. Food & Machinery,2016,32(1):34-37.

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  • 在线发布日期: 2023-02-26
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