不同修饰程度对蜡质玉米淀粉理化性质和消化特性的影响
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(1. 江南大学粮食发酵工艺与技术国家工程实验室,江苏 无锡 214122;2. 江南大学食品学院,江苏 无锡 214122)

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何健(1991—),男,江南大学在读硕士研究生。E-mail: Adrian_HJ@163.com

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国家自然科学基金资助项目(编号:31201381,31301505)


Effect of branch-chain elongation on physicochemical properties and
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(1. National Engineering Laboratory for Cereal Fermentation Technology, Jiangnan University, Wuxi, Jiangsu 214122, China; 2. School of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu 214122, China)

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    摘要:

    利用淀粉蔗糖酶(AS)的转糖基活力修饰蜡质玉米淀粉(WCS),探索不同修饰程度对其理化性质和消化特性的影响。结果表明:在给定的反应条件下,酶反应进程可用对数函数进行模拟;与A型的原淀粉相比,改性WCS的结构更为致密,且呈现出B型结晶特性;改性前后淀粉的支链长度分布变化显著,随着转糖基率(TR)的增加,Fr I(聚合度(DP)>30)组分所占比例显著增加,Fr III(DP<13)组分所占比例显著减小;与原淀粉(抗性淀粉(RS)含量=0.8%)相比,改性WCS的RS含量明显提高(53.1%≤RS含量≤73.6%),随着TR的增加,改性WCS的RS含量呈现先增加后缓慢降低的趋势,在TR=88%时,RS含量达到最高(73.6%)。

    Abstract:

    Waxy corn starch (WCS) was modified by amylosucrase (AS). The effect of starch elongation degree on the digestibility and physicochemical properties of modified waxy corn starch (WCS) was investigated. The results showed that under the given reaction condition, enzymatic reaction could be fitted by a logarithmic mathematical model. Compared with native starch, the modified WCSs expressed a continuous matrix structure, and the packing arrangement of crystalline converted from A-type into B-type. With transglycosylation rate (TR) increasing, an increased tendency of proportion of Fr I (DP>30) was observed, whereas the contents of Fr III (DP<13) was significantly decreased. In vitro test showed that the resistant starches (RS) were conspicuously enhanced in modified WCSs (53.1%≤RS content≤73.6%) compared to raw starch (RS content =0.8%). With the increased TR, the contents of RS in modified WCSs were increased at the beginning and attained the highest content (73.6%) when TR=88%, then RS content slowly declined.

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引用本文

何健,王韧,张昊,等.不同修饰程度对蜡质玉米淀粉理化性质和消化特性的影响[J].食品与机械,2016,32(1):1-5.
HEJian, WANGRen, ZHANGHao, et al. Effect of branch-chain elongation on physicochemical properties and[J]. Food & Machinery,2016,32(1):1-5.

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  • 收稿日期:2015-10-07
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  • 在线发布日期: 2023-02-26
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