Two different cold store models, one with downward blowing fans and the other with back blowing fans, based on a medium practical cold storage were established to investigate the influence of different fans arrangement on airflow in the cold store (24.3 m×21.6 m×7.2 m). Steady-state simulation was performed by using fluent software and stacking cargos were considered as porous medium. Darcy-Forchheimer and ergun equations were used to describe the parameter of stacking cargo combined with former research. Essential analysis and comparison were also performed. The influence of different fans arrangement on stacking cargo and air flow in the cold store were investigated. A uniform velocity and temperature distribution was found in the cold store with down blowing fans. Inhomogeneous temperature distribution was found in the cold store with back blowing fans. The result of simulations showed that the uniformity of air distribution in the cold store with downward blowing fans performed better than the one with back blowing fans. High temperature region was found in the cargo area at the remote end of blowing fan. Therefore, at least in this case, conclusion can be made that downward blowing was better than that of back blowing, not only in predicted velocity and temperature value but also in the uniformity.