A method was developed for simultaneous determination of 8 β-lactams in goat milk by QuEChERS-ultra performance liquid chromatography-tandem mass spectrometry (UPLC—MS/MS). The milk was extracted with acetonitrile, purified with C₁₈ and PSA sorbent and separated by an ACQUITY UPLC BEH C₁₈ column using water (containing 0.1% formic acid) and acetonitrile as mobile phase. The mass spectrometric acquisitions were carried out by means of multiple reaction monitoring (MRM) in positive ionization mode. The linear relation of eight β-lactams was good and the correlation coefficient was more than 0.986. The recovery rates of the target analyte were between 83.8%～95.4%. The relative standard deviations were less than 6.5%. The limit of detection (LOD) of 8 β-lactams was 0.25～1.00 μg/kg and the limit of quantification (LOQ) was 1.0～2.0 μg/kg. This method was reliable for the determination of β-lactams in goat milk and other dietary products.