苦苦菜发酵过程中主要发酵菌种及滋味物质的变化
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(1. 宁夏大学农学院,宁夏 银川 750021;2. 宁夏大学生命科学学院,宁夏 银川 750021;3. 平罗县农副产品流通服务中心,宁夏 石嘴山 753400)

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张婷(1990—),女,宁夏大学农学院在读硕士研究生。E-mail:ztgl1122@126.com

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宁夏回族自治区科技支撑计划项目(编号:2012zyn102)


Study on the main fermentation strains and taste substances in fermentation of dandelion
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(1. College of Agricultural, Ningxia University, Yinchuan, Ningxia 750021, China; 2. College of Life Science, Ningxia University, Yinchuan, Ningxia 750021, China; 3. Pingluo Agricultural and Sideline Products Circulation Service Center, Shizuishan, Ningxia 753400, China)

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    摘要:

    以苦苦菜为原料进行腌制,对自然发酵的苦苦菜进行菌种分离鉴定和总酸度、pH及盐度的测定。游离氨基酸含量用氨基酸自动分析仪分析,并按其味觉强度进行分类。结果表明:苦苦菜发酵初期以植物乳杆菌为优势乳酸菌,呈味氨基酸中苦味氨基酸(Leu、Val、Arg、Ile、Trp、Met)含量较高。主发酵期以植物乳杆菌和短乳杆菌为优势菌群,甜味氨基酸(Ser、Thr、Gly、Ala、His、Pro) 和鲜味氨基酸(Glu、Asp、Lys) 的含量逐渐增加,而苦味氨基酸含量逐渐降低。发酵后期以发酵乳杆菌为优势乳酸菌,甜味和鲜味氨基酸含量均高于苦味氨基酸。整个发酵过程中芳香族氨基酸(Tyr、Cys、Phe)含量较低且变化不大,发酵中后期7~20 d苦苦菜的滋味最好。

    Abstract:

    With dandelion as raw materials, the bacteria were isolated and identified , and the total acid, pH and salinity were also identified from the naturally fermented product. Free amino acids were analyzed by amino acid analyzer and were classified in accordance with the taste intensity. The results showed that Lactobacillus planetarium was dominant and the content of bitter amino acids (Leu, Val, Arg, Ile, Trp, Met) was higher than the others in the early fermentation process. Lactobacillus planetarium and Lactobacillus brevis took an active part in the main fermentation stage. Meanwhile, the content of sweet amino acids (Ser, Thr, Gly, Ala, His, Pro) and umami amino acids (Glu, Asp, Lys) increased but the bitter amino acids' content decreased. In the late fermentation process, Lactobacillus fermentum was dominant, and the sweet and umami amino acids' content was higher than the bitter. Throughout the fermentation process, the content of aromatic amino acids (Tyr, Cys, Phe) changed a little. Dandelion enjoyed a best taste from the seventh to twentieth day after fermentation.

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张婷,杨波,罗瑞明,等.苦苦菜发酵过程中主要发酵菌种及滋味物质的变化[J].食品与机械,2015,31(3):23-27.
ZHANGTing, YANGBo, LUORuiming, et al. Study on the main fermentation strains and taste substances in fermentation of dandelion[J]. Food & Machinery,2015,31(3):23-27.

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  • 收稿日期:2015-03-08
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  • 在线发布日期: 2023-03-17
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