Objective Phellinus igniarius is a kind of rare medicinal fungi, its flavone can be used as medicine. This research was to optimize liquid culture medium for flavone fermention.Methods Taking the mycelia flavone yield of Phellinus igniarius as index, the liquid culture medium was optimized with L₉(3⁴) orthogonal test. Results The optimal medium for high flavone yield established was A₃B₃C₂D₂: corn flour 3%+ wheat bran 7%, peptone 2%, KH₂PO₄ 0.10%, MgSO₄ 0.15%, mycelia flavone yield of Phellinus igniarius was 212.35 mg/L. Conclusion Liquid culture medium optimized will have certain significance for guiding flavone’s industrialized production.