To study the anti-tumor activities of wild edible mushrooms of the Changbai Mountain, the experiment selected Ganoderma Lucidum, Inonotus Obliquus, Phellinus, Hericium Erinaceus, Lentinula Edodes five kinds of Changbai Mountain wild edible mushrooms as materials, the experiment was divided into in vitro and in vivo study. The inhibition effect of tumor cells was tested by MTT method, and the inhibition ratio of mouse sarcoma S-180 was calculated in vivo study. The results showed that Ganoderma Lucidum has the highest inhibition ratio on the HeLa and HepG2 cell lines. Phellinus has the highest inhibition rate on MCF-7 cell line, Hericium Erinaceus showed the strongest inhibitory effect on the A549 cell lines in vitro study. And the other kinds of wild edible mushrooms displayed obviously dose-dependent inhibitory effect but except Ganoderma Lucidum. In vivo study, all the wild edible mushrooms showed the tumor suppression effect in mouse sarcoma S-180 cell line, which the maximum, high dose group of Hericium Erinaceus was 75.92%, but the lowest doses of Lentinula Edodes extract has showed high tumor inhibition rate (69.39%).