四川产区蓝莓病原真菌的分离鉴定及精油成分对其的抑菌作用
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刘春燕,女,浙江海洋大学在读硕士研究生。

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四川省科技计划(编号:2021JDRC0030,2021YJ0262);干热河谷特色生物资源开发(编号:GR-2020-E-01);四川省大学生创新创业训练计划(编号:S202111552110)


Isolation and identification of pathogenic fungi from postharvest blueberry in Sichuan province and the inhibition effect on them by using essential oil
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    摘要:

    目的:控制四川产区蓝莓采后的真菌性病害。方法:以四川省14个种植基地的蓝莓果实为试材,进行病原真菌分离鉴定、致病性测定;针对蓝莓采后优势和亚优势菌株,研究温度和光照对菌丝生长的影响,及有效天然抑菌成分筛选。结果:引起蓝莓采后腐烂的病原真菌有Q1W1(Aspergillus niger)、Q1W11(Aspergillus tubingensis)、Q2W13(Neofusicoccum parvum)、Q2W19(Neopestalotiopsis sp. )、Q2W20(Fusarium graminearum)、Q2W22(Alternaria alternata)、Q2W26(Mucor fragilis)、Q3W7(Penicillium sp.)、Q3W25(Cladosporium xylophilum)9种。其中Q1W1是优势菌株,分离频率最高,致病性最强;Q2W19、Q2W22、Q3W7和Q3W25是亚优势菌株,分离频率大于10%。Q1W1、Q2W19、Q2W22、Q3W7和Q3W25的最适生长温度范围为20~35 ℃。光照对Q1W1、Q2W19、Q2W22和Q3W25的菌丝生长有显著影响,对Q3W7的无影响。15种精油组分中以麝香草酚的广谱抑菌活性最好,其对蓝莓采后优势和亚优势菌株的EC50范围为30~50 mg/L,而麝香草酚水溶液直接用于蓝莓采后真菌防治的实际施用浓度不应高于50 mg/L。结论:麝香草酚对四川产区蓝莓采后病害的抑制效果较好。

    Abstract:

    Objective:This study aimed to provide strategy for controlling the fungal diseases of postharvest blueberry in Sichuan province.Methods:The pathogenic bacteria from 14 planting bases in Sichuan Province were isolate, identified, pathogenicity determination. Moreover, the effects of temperature and light on mycelia growth of the dominant and subdominant strains of blueberry after harvest were studied, and the effective natural antibacterial components against them were screened.Results:The blueberry postharvest rot diseases were caused by the following nine kinds of fungi:Aspergillus niger,Aspergillus tubingensis,Neofusicoccum parvum,Neopestalotiopsissp.,Fusarium graminearum,Alternaria alternata,Mucor fragilis,Penicilliumsp.,Cladosporium xylophilum.Aspergillus niger was the dominant species with the highest isolation frequency and the most pathogenic, andNeopestalotiopsissp.,Alternaria alternata,Penicilliumsp.,Cladosporium xylophilum were the subdominant species with isolation frequencies greater than 10%. The optimum growth temperature ranged from 20 ℃to 35 ℃of dominant and subdominant strains of blueberry postharvest. The effects of light on the mycelia growth ofA. niger,Neopestalotiopsissp., A. alternata,and C. xylophilum were significant,but that was insignificant on the growth ofPenicilliumsp.strains. Thymol showed good broad-spectrum antibacterial activity, the EC50 values of dominant and subdominant strains of blueberry postharvest was 30~50 mg/L, while the thymol applied directly to blueberry postharvest fungal control in aqueous solution should not be higher than 50 mg/L.Conclusion:Thymol is more effective on postharvest diseases of blueberry in Sichuan province.

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刘春燕,丁捷,邓尚贵,等.四川产区蓝莓病原真菌的分离鉴定及精油成分对其的抑菌作用[J].食品与机械,2022,(4):134-140.
LIU Chun-yan, DING Jie, DENG Shang-gui, et al. Isolation and identification of pathogenic fungi from postharvest blueberry in Sichuan province and the inhibition effect on them by using essential oil[J]. Food & Machinery,2022,(4):134-140.

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  • 在线发布日期: 2022-07-20
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