There were two methods to detect the plasticizers of red wine. The first one was a traditional method(GC-MS) and another one was a new method-ELISA kit. An improved pre-processing of GC-MS and the comparative analysis of the two methods was used to find a novel detection method. The results: there was a little difference for the rate of plasticizer extraction with different pre-treatment. And DIBP, DBP, DEHP were detected. The GC-MS method demonstrated high precision and recovery. The GC-MS method was a excellent method to detect the plasticizers of red wine. The detection results of ELISA kit were 3.4 to 5.5 times larger than the GC-MS method. It was enough for ELISA kit as a rapid detection method of the plasticizers of red wine.