The sample was purifid by solid phase extraction column. A simple, fast, accurate method for determination of Imibenconazole in fruits and vegetables was developed by ultra high performance liquid chromatography-tandem quadrupole mass spectrometry (UPLC—MS—MS). The linear range of the method is from 1.0 μg/L to 100 μg/L ，and the correlation coefficients were 0.998 7～0.999 6, and the detection limit is 1.0 μg/kg. The proposed method has high sensitivity and good reproducibility for determining Imibenconazole.